pET28-N-His-thromb
Expression vector with T7 promoter, adds N-terminal His tag, thrombin site; kanamycin resistance; restriction enzyme cloning.
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Cutters
Show ORFs
| Name | Type | Location | Length |
|---|---|---|---|
| ColE | 1421 to 2103 | 683 bp | |
| reverse | 5885 to 5861 | 25 bp | |
| T7 | 4987 to 5006 | 20 bp | |
| LacI | 3515 to 4531 | 1017 bp | |
| KanR | 563 to 1375 | 813 bp | |
| His | 5847 to 5864 | 18 bp | |
| lac operator | 5006 to 5028 | 23 bp |
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GC Content:
Location:
-
Length:
-
Identity:
Manage Enzymes
| Enzyme | Cuts |
|---|---|
| AatII | 0 |
| Acc65I | 0 |
| AccI | 0 |
| AciI | 0 |
| AclI | 0 |
| AcuI | 0 |
| AfeI | 0 |
| AflII | 0 |
| AflIII | 0 |
| AgeI | 0 |
| AhdI | 0 |
| AleI | 0 |
| AluI | 0 |
| AlwI | 0 |
| AlwNI | 0 |
| ApaI | 0 |
| ApaLI | 0 |
| ApeKI | 0 |
| ApoI | 0 |
| AscI | 0 |
| AseI | 0 |
| AsiSI | 0 |
| AvaI | 0 |
| AvaII | 0 |
| AvrII | 0 |
| BaeGI | 0 |
| BaeI | 0 |
| BamHI | 0 |
| BanI | 0 |
| BanII | 0 |
| BbsI | 0 |
| BbvCI | 0 |
| BbvI | 0 |
| BccI | 0 |
| BceAI | 0 |
| BcgI | 0 |
| BciVI | 0 |
| BclI | 0 |
| BfaI | 0 |
| BfuAI | 0 |
| BfuCI | 0 |
| BglI | 0 |
| BglII | 0 |
| BlpI | 0 |
| BmgBI | 0 |
| BmrI | 0 |
| BmtI | 0 |
| BpmI | 0 |
| Bpu10I | 0 |
| BpuEI | 0 |
| BsaAI | 0 |
| BsaBI | 0 |
| BsaHI | 0 |
| BsaI | 0 |
| BsaJI | 0 |
| BsaWI | 0 |
| BsaXI | 0 |
| BseRI | 0 |
| BseYI | 0 |
| BsgI | 0 |
| BsiEI | 0 |
| BsiHKAI | 0 |
| BsiWI | 0 |
| BslI | 0 |
| BsmAI | 0 |
| BsmBI | 0 |
| BsmFI | 0 |
| BsmI | 0 |
| BsoBI | 0 |
| Bsp1286I | 0 |
| BspCNI | 0 |
| BspDI | 0 |
| BspEI | 0 |
| BspHI | 0 |
| BspMI | 0 |
| BspQI | 0 |
| BsrBI | 0 |
| BsrDI | 0 |
| BsrFI | 0 |
| BsrGI | 0 |
| BsrI | 0 |
| BssHII | 0 |
| BssKI | 0 |
| BssSI | 0 |
| BstAPI | 0 |
| BstBI | 0 |
| BstEII | 0 |
| BstNI | 0 |
| BstUI | 0 |
| BstXI | 0 |
| BstYI | 0 |
| BstZ17I | 0 |
| Bsu36I | 0 |
| BtgI | 0 |
| BtgZI | 0 |
| BtsCI | 0 |
| BtsI | 0 |
| Cac8I | 0 |
| ClaI | 0 |
| CspCI | 0 |
| CviAII | 0 |
| CviKI_1 | 0 |
| CviQI | 0 |
| DdeI | 0 |
| DpnI | 0 |
| DpnII | 0 |
| DraI | 0 |
| DraIII | 0 |
| DrdI | 0 |
| EaeI | 0 |
| EagI | 0 |
| EarI | 0 |
| EciI | 0 |
| Eco53kI | 0 |
| EcoNI | 0 |
| EcoO109I | 0 |
| EcoRI | 0 |
| EcoRV | 0 |
| FatI | 0 |
| FauI | 0 |
| Fnu4HI | 0 |
| FokI | 0 |
| FseI | 0 |
| FspI | 0 |
| HaeII | 0 |
| HaeIII | 0 |
| HgaI | 0 |
| HhaI | 0 |
| HinP1I | 0 |
| HincII | 0 |
| HindIII | 0 |
| HinfI | 0 |
| HpaI | 0 |
| HpaII | 0 |
| HphI | 0 |
| Hpy166II | 0 |
| Hpy188I | 0 |
| Hpy188III | 0 |
| Hpy99I | 0 |
| HpyAV | 0 |
| HpyCH4III | 0 |
| HpyCH4IV | 0 |
| HpyCH4V | 0 |
| KasI | 0 |
| KpnI | 0 |
| MboI | 0 |
| MboII | 0 |
| MfeI | 0 |
| MluI | 0 |
| MlyI | 0 |
| MmeI | 0 |
| MnlI | 0 |
| MscI | 0 |
| MseI | 0 |
| MslI | 0 |
| MspA1I | 0 |
| MspI | 0 |
| MwoI | 0 |
| NaeI | 0 |
| NarI | 0 |
| NciI | 0 |
| NcoI | 0 |
| NdeI | 0 |
| NgoMIV | 0 |
| NheI | 0 |
| NlaIII | 0 |
| NlaIV | 0 |
| NmeAIII | 0 |
| NotI | 0 |
| NruI | 0 |
| NsiI | 0 |
| NspI | 0 |
| PacI | 0 |
| PaeR7I | 0 |
| PciI | 0 |
| PflFI | 0 |
| PflMI | 0 |
| PhoI | 0 |
| PleI | 0 |
| PmeI | 0 |
| PmlI | 0 |
| PpuMI | 0 |
| PshAI | 0 |
| PsiI | 0 |
| PspGI | 0 |
| PspOMI | 0 |
| PspXI | 0 |
| PstI | 0 |
| PvuI | 0 |
| PvuII | 0 |
| RsaI | 0 |
| RsrII | 0 |
| SacI | 0 |
| SacII | 0 |
| SalI | 0 |
| SapI | 0 |
| Sau3AI | 0 |
| Sau96I | 0 |
| SbfI | 0 |
| ScaI | 0 |
| ScrFI | 0 |
| SexAI | 0 |
| SfaNI | 0 |
| SfcI | 0 |
| SfiI | 0 |
| SfoI | 0 |
| SgrAI | 0 |
| SmaI | 0 |
| SmlI | 0 |
| SnaBI | 0 |
| SpeI | 0 |
| SphI | 0 |
| SspI | 0 |
| StuI | 0 |
| StyD4I | 0 |
| StyI | 0 |
| SwaI | 0 |
| TaqI | 0 |
| TfiI | 0 |
| TliI | 0 |
| TseI | 0 |
| Tsp45I | 0 |
| Tsp509I | 0 |
| TspMI | 0 |
| TspRI | 0 |
| Tth111I | 0 |
| XbaI | 0 |
| XcmI | 0 |
| XhoI | 0 |
| XmaI | 0 |
| XmnI | 0 |
| ZraI | 0 |
Restriction Digest
One
| Enzyme | Cuts |
|---|
None selected
Two
| Enzyme | Cuts |
|---|
None selected
Three
| Enzyme | Cuts |
|---|
None selected